Mean infective dose in cultured cells per milimeter was estimated by reed and muench. Examples include ld50 the median lethal dose of a toxin or pathogen, ec50. Two commonly used methods for calculating 50% endpoint using serial dilutions are spearmankarber method and reed and muench method. Determination of 50% endpoint titer using a simple formula. See article above for overview of 50% endpoints and comparison with other methods of calculating 50% endpoints. May 12, 2016 here, i have proposed a simple formula to calculate the 50% endpoint titre and this formula can be used in addition to reed and muench or spearmankarber, methods but not exclusively at this point. Determination of 50% endpoint titer using a simple formula ncbi. In the following section, the newly proposed method is compared with two commonly used methods viz. The reedmuench method is a simple method for determining 50% endpoints in experimental biology, that is, the concentration of a test substance that produces an effect of interest in half of the test units. Usda center for veterinary biologics statistics section usda aphis. Similar books metodo reed muench pdf free reed and muench 1938 pdf free reed and muench free download pdf reed and muench reed muench reed muench calculator tcid50 reed muench reed muench method calculation the broken reed saxophone quartet a reed breaks in. All books are the property of their respective owners. The reedmuench method is a simple method for determining 50% endpoints in. The reedmuench and dragstedtbehrens estimators are largely of historical interest.