Qiaquick gel extraction kit 50 from qiagen sample to. Qiaprep spin miniprep kit highyield protocol english pdf. The use of a dedicated kit for this application may be necessary, since kits can vary depending on the type of reaction and dna fragment size e. Monarch nucleic acid purification kits are optimized for maximum performance and minimal environmental impact. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit.
Purification of total dna from plant tissue mini protocol 22 purification of total dna from plant tissue maxi protocol 26. Laboratory protocol for dna extraction from oracollectdx. Qiavac vacuum manifolds 30 handling guidelines for qiavac 6 31 qiavac 24 32 ordering information 33 qiagen companies and distributors 35 qiaquick spin handbook 072002 3. Qiagen qiaquick gel extraction kit, 50 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10g dna 70. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of between 20 and 100 l. Qiaquick gel extraction kit 50 from qiagen sample to insight. Comparison of rna yields from 30 mg of mouse liver, heart, and brain tissue. Kessler for our clinical studies, we utilize qiagens dneasy blood and tissue kit to extract dna from dried blood spots.
Qiaamp dna mini and blood mini handbook moodle ufsc. Up to 400 mg agarose can be processed per spin column. The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for molecular biology applications. Im using the qiagen rneasy plus mini kit for rna extraction from brain tissue and adipose tissue. This protocol is designed for purification of total dna from grampositive bacteria. Qiaquick gel extraction kit protocol using a microcentrifuge. Qiagen plasmid purification handbook harvard university. Gentra puregene handbook 062011 5 kit contents plus gentra puregene blood kit 3 ml 30 ml 120 ml ml ml catalog no. For maximum convenience and value, columns and buffers are also available separately. We recommend all users of qiagen products to adhere to the nih guidelines. Pdf a simplified universal genomic dna extraction protocol.
Many labs use dnarna extraction kits to isolate total rna or dna from a wide variety of samples, including animal and plant cells and tissue, bacteria and yeast. Dna extraction protocol for iswabdna using qiaamp dna mini. Qiagen provides a variety of qiaamp genomic dna kits for fast, efficient and easy dna purification figures 1 and 2. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Automated dna cleanup the minelute pcr purification kit and minelute gel extraction kit can be fully automated on the qiacube. A simplified universal genomic dna extraction protocol. Dneasy plant handbook 102012 3 contents kit contents 4. Purelink quick plasmid miniprep kit purelink hipure plasmid miniprep kit purelink pro quick96 plasmid kit magjet plasmid dna protocol time rnadna mini kit provides a rapid and efficient method to simultaneously purify viral rnadna from fresh or frozen cellfree biological fluids plasma, serum, cerebrospinal fluid and cell. Purification of total dna from plant tissue mini protocol 22. Dna purification from dried blood spots qiaamp dna mini kit. The kit can be used to purify nucleic acids from a. For purification of lowcopy plasmids and cosmids, large plasmids 10 kb, and dna prepared using other.
Purelink viral rnadna mini kit thermo fisher scientific. The kit uses qiagens secondgeneration inhibitor removal technology irt, and is intended for use with. In this stepbystep dneasy visual protocol, youll see how to extract genomic dna from various samples in just 20 minutes. For all tissue types, the purelink kit yielded more rna than the qiagen kit. Qiaprep spin miniprep kit protocol using a microcentrifuge this protocol is designed for purification of up to 20 g of highcopy plasmid dna from 15ml overnight cultures of liin lb luriabertani medium. The purelink viral rnadna mini kit provides a rapid and efficient method to simultaneously purify viral rnadna from fresh or frozen cellfree biological fluids plasma, serum, cerebrospinal fluid and cell culture supernatants. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. They can be stored for at least 2 years without showing any reduction in performance, capacity, or quality of separation. Qiaamp dna micro handbook 082003 7 introduction the qiaamp dna micro kit uses wellestablished technology for purification of genomic and mitochondrial dna from small sample volumes or sizes. Forceps are to be sterilized in fine science tools heat block at. Qiagen allprep dna rna ffpe kit protocol pugh lab printed.
These products are not intended for the diagnosis, prevention, or treatment of a disease. Purify highquality, genomic dna from a broad range of sample types cells, blood, tissues, and more. Protocol qiaex ii agarose gel extraction protocol this protocol is designed for the extraction of 40bp to 50kb dna fragments from 0. Rna, proteins, dyes, and lowmolecularweight impurities are removed by a mediumsalt wash. While the kits are low hazard on their own, its important to remember that mixing incompatible chemicals can produce high hazard byproducts and gases.
Qiaamp dna mini and blood mini handbook 112007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use. Qiagen plasmid purification protocols are based on a modified alkaline lysis procedure, followed by binding of plasmid dna to qiagen anionexchange resin under appropriate lowsalt and ph conditions. Kessler for our clinical studies, we utilize qiagen s dneasy blood and tissue kit to extract dna from dried blood spots on whatman fta cardsfilter papers. The brief version of the procedure is below for reference. Purification of total dna from animal tissues spincolumn protocol this protocol is designed for purification of total dna from animal tissues, including rodent tails. The kit includes lysis buffers for various input materials, proteinase k for homogenization of some sample types, and rnase a. All due care and attention should be exercised in the handling of the products. These are available online in convenient and compact pdf format.
Protocol for extraction and purification of genomic dna from. This kit can also be used for dna cleanup from enzymatic reactions see page 8. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. The rneasy mini kit, rneasy protect mini kit including rna later rna stabilization reagent, and rneasy plant mini kit should be stored dry at room temperature 1525c and are stable for at least 9 months under these conditions. Protocol for extraction and purification of genomic dna. Lyophilized qiagen protease can be stored at room temperature 1525c for up to 6 months without any decrease in performance.
Dna isolated with the dna extraction kit is free from contaminants and may be used directly for restriction digests, cloning, southern blotting, pcr amplification, and other dna analysis techniques. The innovative qiacube uses advanced technology to process qiagen. In accordance with qiagens isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to. Kits are available for total rna purification, plasmid miniprep, gel extraction. A simplified universal genomic dna extraction protocol suitable for pcr article pdf available in genetics and molecular research. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. Ethanol precipitation is never required and purified dna is immediately ready for. Dneasy blood and tissue kit 50 from qiagen sample to. These are available online in convenient and compact pdf format at. Purelink quick plasmid miniprep kit purelink hipure plasmid miniprep kit purelink pro quick96 plasmid kit magjet plasmid dna protocol time dna purification kit is a universal kit for dna extraction and purification from a wide variety of cell types, including blood, cells, tissues and toughtolyse samples including bacteria and yeast. The dna extraction kit1 is a modification of a procedure based on separating contaminating protein from dna by salt precipitation. Qiaamp dna mini and blood mini handbook moodle ufsc moodle. Qiagen allprep dnarna ffpe kit protocol pugh lab printed.
For gel extractioncleanup of up to 10 g dna 70 bp to 10 kb from enzymatic reactions. The monarch genomic dna purification kit provides excellent yields of higher quality, higher. Qiaex ii handbook qiaex ii agarose gel extraction protocol. The purelink viral mini kit is specifically designed to isolate highq. Qiaquick nucleotide removal kit protocol 21 qiaquick gel extraction kit protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 troubleshooting guide 28 appendix. Add ethanol 96100% to buffer pe before use see bottle label for volume. Wheeler lab protocols dna extraction qiagen dneasy kit. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. Follow the dna purification from blood or body fluids spin protocol procedure in the qiaamp dna mini kit handbook qiagen cat. Purification of total dna from animal tissues spin. For liver and brain tissue, the purelink kit recovered approximately twice as much rna as the qiagen kit.